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KMID : 0624620120450030165
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BMB Reports
2012 Volume.45 No. 3 p.165 ~ p.170
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Identification of anti-HIV and anti-Reverse Transcriptase activity from Tetracera scandens
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Kwon Hyeok-Sang
Park Jung-Ae
You Ji-Chang
Kim Joo-Hwan
You Ji-Chang
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Abstract
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We report here that an ethanol extract of Tetracera scandens, a Vietnamese medicinal plant, has anti-HIV activity and possesses strong inhibitory activity against HIV-1 reverse transcriptase (RTase). Using a MT-4 cell-based assay, we found that the T. scandens extract inhibited effectively HIV virus replication with an IC(50) value in the range of 2.0-2.5 μg/ml while the cellular toxicity value (CC50) was more than 40-50 μg/ml concentration, thus yielding a minimum specificity index of 20-fold. Moreover, the anti-HIV efficacy of the T. scandens extract was determined to be due, in part, to its potent inhibitory activity against HIV-1 RTase activity in vitro. The inhibitory activity against the RTase was further confirmed by probing viral cDNA production, an intermediate of viral reverse transcription, in virus-infected cells using quantitative DNA-PCR analysis. Thus, these results suggest that T. scandens can be a useful source for the isolation and development of new anti- HIV-1 inhibitor(s).
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KEYWORD
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Anti-HIV agent(s), Anti-HIV Reverse Transcriptase, HIV-1, Tetracera scandens
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